What is cryopreservation? It’s a method of preserving tissues, cells, organelles, or any other biological materials by drastically lowering the temperature of the samples.
The way living cells respond to the formation of ice is both a topic of practical relevance and theoretical interest. Researchers cannot store stem cells or other viable tissues (for general research and medical applications) for long periods of time. Ice crystals, membrane damage, and osmotic shock due to freezing and thawing kills these cells.
Fortunately, in recent years, efforts at cryopreservation with various cryoprotective agents (CPAs) and temperature-controlled equipment have proven increasingly successful. It’s critical to understand the chemical and physical reactions which occur during freezing and thawing to further the cryopreservation of cells.